![]() Stable Markers for Northern Analysis - TechNotes 8(4)ĭescription: Markers provided in formamide for increased RNase resistance. ![]() Premade, RNase-free Northern Reagents - TechNotes 9(1)ĭescription: Increase Northern sensitivity by limiting degradation of probe and of RNA samples bound to your blots Northern Analysis: Faster and Better - TechNotes 11(2)ĭescription: Decrease hybridization and exposure time with ULTRAhyb® Ultrasensitive Hybridization Buffer. Get More From Your Blots with ULTRAhyb® Technology - TechNotes 14(2)ĭescription: Here we show that by selecting a more sensitive hybridization buffer, the percentage of target molecules that hybridize to probe on a Northern blot can be significantly increased. Ten Ways to Increase the Sensitivity of Northern Hybridizationsĭescription: Reduce Northern blot exposure times from days to hours by pushing blot hybridizations towards completion First, one extracts the RNA from embryos at different stages of development, or from different organs of the same embryo. If you have never performed Northern analysis, or if you want to optimize your Northern blot experiments, you will definitely want to check out this informative article. We can determine the temporal and spatial locations where RNAs are expressed by running an RNA blot (often called a Northern blot). This protocol describes an improved northern blot method that enhances detection of small RNA molecules (<40 nt) including regulatory species such as microRNA (miRNA), short-interfering RNA (siRNA. ![]() All of these can be used to confirm results from microarray analysis and differential display experiments.Ĭomparison of Different Methods of mRNA Quantitationĭescription: A brief comparison of nuclease protection assays, Northern blotting, RT-PCR and in situ hybridization for the detection and quantitation of RNA.ĭescription: "Northern Analysis: The Basics" leads you through the steps involved in Northern blotting and explains the considerable improvements Ambion has found over standard Northern blotting protocols. This protocol describes the detection of small RNAs (10200 nucleotides) by blot hybridization. Includes recommendations for both radiolabeled and nonisotopically labeled DNA, RNA and oligonucleotide probes.Īnalysis of Differential Gene Expression - Technical Bulletin #500ĭescription: Here, we review three popular RNA detection/quantitation methods: Northern blot analysis, Ribonuclease Protection Assays (RPAs) and Reverse Transcription Polymerase Chain Reaction (RT-PCR). Amount of Probe to Use in a Northern Blotĭescription: A quick chart of various probe concentrations to use in your Northern blotting experiments.
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